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KMID : 0545120130230040483
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Journal of Microbiology and Biotechnology
2013 Volume.23 No. 4 p.483 ~ p.488
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Production of Ginsenoside Rd from Ginsenoside Rc by α-L-Arabinofuranosidase from Caldicellulosiruptor saccharolyticus
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Shin Kyung-Chul
Lee Gi-Woong
Oh Deok-Kun
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Abstract
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Ginsenoside Rd was produced from ginsenoside Rc using a thermostable recombinant α-L-arabinofuranosidase from Caldicellulosiruptor saccharolyticus. The optimal reaction conditions for the production of ginsenoside Rd from Rc were pH 5.5, 80oC, 227 U enzyme/ml, and 8.0 g/l ginsenoside Rc in the presence of 30% (v/v) n-hexane. Under these conditions, the enzyme produced 7.0 g/l ginsenoside Rd after 30 min, with a molar yield of 100% and a productivity of 14 g l-1 h-1. The conversion yield and productivity of ginsenoside Rd are the highest reported thus far among enzymatic transformations.
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KEYWORD
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Ginsenoside Rd, ginsenoside Rc, biotransformation, α-L-arabinofuranosidase, Caldicellulosiruptor saccharolyticus
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